Genetic analysis of salmon sushi and sashimi traded in Belo Horizonte - MG Brazil

Mariana Cotta Vieira

Abstract


Genetics has enabled the identification of the species and can be used as a comparison tool for various purposes. Especially for species identification through DNA analysis. The introduction of DNA barcoding highlighted expanding the use of mitochondrial DNA in the subunit I of cytochrome c oxidase (COI) as a genetic marker for identifying individuals (Dawney et al., 2007). It was used barcode code of Life Data Systems (BOLD), the adoption of the DNA barcode technology as the "gold standard" for species identification. The BOLD database provides detailed information on species with sequenced the COI gene, including the origin and current location of testimony specimens. The target species of this work is the salar salmon and aimed to analyze the degree of reliability in salmon sold in Belo Horizonte (MG). Samples identified as salmon were purchased in five different commercial establishments in the city of Belo Horizonte - MG. The DNA was extracted using the 'salting out DNA extraction by' protocol (Miller SA, et al., 1988). The measurement of CRP was only possible in the sample 3A which was collected from the EPA supermarket, located in the New Town neighborhood, Belo Horizonte (MG), then the procedure was repeated and again was not successful in the other samples. It is important that the government of all countries enforce fraud in fish, improving existing laws and the coordination of information between federal agencies, so that knowledge of fish origin is readily available to the consumer. This study is essential to complement the taxonomy of species and for a number of forensic analysis thereof.

 

Keywords: Salmo salar. Fishing Trade. COI gene.


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